The main experimental facilities of the department are the laboratories. The genetic analysis laboratory has been developing since the 1990s. From the financial resources obtained as part of the resolved investment A - projects of the MSM, research plans and development projects of the Faculty, investment equipment was gradually secured, which is used for the acquisition and implementation of research grant projects and at the same time for the education and training of students in bachelor's, master's and doctoral programs. In the past years, several trainings for employees of collaborating research institutions have been organized within the laboratory.
Currently, the laboratory is equipped to carry out complex genetic analysis, from DNA analysis based on PCR, SSR and mutation detection by SSCP, DGGE and Sanger sequencing methods to quantitative analysis at the DNA or RNA level.
Significant milestones in the development of laboratory
2020
A significant shift towards quantitative analyses. Thanks to the FAPPZ contribution, the department received a significant investment: a droplet digital PCR and a large-capacity RealTime platform for the implementation of the necessary quantitative genetic analyses. The way to new projects is open.
2016
As a result of the completion of the MCEV II and Pavilion D buildings, the laboratory was expanded by two sub-laboratories in the sector of the Department of Genetics and Breeding in the main building of FAPPZ focused on RNA isolation and analysis. Established methods based on the RealTime PCR principle still use Light Cycler device.
2013
By purchasing the Light Cycler device, we expand the equipment and the possibility of using the RealTime PCR method. This year, the AFLP method was successfully optimized: "on the knee" - without the use of a commercial kit.
2010
Equipping the workplace with ABI Prism 310 single-capillary electrophoresis - creation of the Laboratory for Fragmentation Analysis and DNA Sequencing.
2006
A significant innovation of equipment from the MSM investment project with the techniques of searching for mutations using the SSCP and DGGE methods with the DCode system and complex evaluation of polymorphisms with the GelDoc XR system from Bio-Rad.
2001
The transition to kit DNA extraction accelerated and greatly simplified the work and made the solution of diploma theses available to "non-chemists" as well. The transition to microvolume PCR brought savings and increased efficiency in solving research tasks
till 2001
We implemented molecular analyzes of DNA based on PCR methods evaluated by gell electrophoresis into daily work. They are routinely used by staff and students of Msc. and PhD. study programs.
